Buffered Hypotonic Solution

Buffered Hypotonic Solution

Buffered Hypotonic Solution is a hypotonic solution developed for bone marrow specimens. It has been found to achieve good metaphase swelling (spreading) from a wide range of sample types and significantly increases the number of useful metaphases in leukaemic bone marrow cultures when compared to the standard 0.075M KCL hypotonic. It contains 0.4% KCL with HEPES.

Buffered Hypotonic Solution is a hypotonic solution developed for bone marrow specimens. It has been found to achieve good metaphase swelling (spreading) from a wide range of sample types and significantly increases the number of useful metaphases in leukaemic bone marrow cultures when compared to the standard 0.075M KCL hypotonic. It contains 0.4% KCL with HEPES.



Applications & Benefits
Developed for the most challenging samples
Two products - Optimal and Buffered Hypotonic
Work on tumour and bone marrow samples or any other samples with very 'tight' metaphases eg keratinocyte, problem blood, rhinoceros(!) etc
Excellent improvements of cytogenetic stem cell preparations
Buffered Hypotonic Solution is a hypotonic solution developed for bone marrow specimens. It has been found to achieve good metaphase swelling (spreading) from a wide range of sample types and significantly increases the number of useful metaphases in leukaemic bone marrow cultures when compared to the standard 0.075M KCL hypotonic. It contains 0.4% KCL with HEPES.


Ordering Information
Buffered Hypotonic Solution - Powder for 250 ml solutionGGS-JL006/a48,00 €
Buffered Hypotonic Solution - Powder for 1000 ml solutionGGS-JL006/b149,00 €

Technical Specification

Procedure

see ordering details
1.Add the powdered contents of the supplied bottle of Buffered Hypotonic Solution product to 1000 mls of distilled water.
2.Adjust to pH 7.4 with 5N NaOH and store at 4°C in a clean glass bottle, until ready to use, noting down the date on the bottle.
3.During harvest replace your existing hypotonic solution with Buffered Hypotonic Solution
4.Replace your existing hypotonic and incubate cells at 37°C for a maximum of 30 minutes.
Applications & Benefits
Ordering Information
Technical Specifications




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PDF-FileProCell_Brochure.pdf
Brochure
0.49 MB
PDF-FileProcell A4 leaflet.pdf
Flyer
1.88 MB
PDF-FileBufferedHypotonicSol...
AppNote
0.44 MB
PDF-FileBuffHypotonicSol_Pow...
MSDS
0.19 MB
PDF-FileBuffHypotonicSol_Pow...
MSDS
0.19 MB


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