Erythrocyte Lysis Solution (ELS)

Erythrocyte Lysis Solution (ELS)

Erythrocyte Lysis Solution is a simple pretreatment of heavily blood-stained amniotic fluid samples which quickly removes heavy red blood cell (erythrocyte) contamination. Unlike many similar products used in molecular biology that lyse red blood cells, this product is extremely gentle in its action and is specially suited for cell culture work. It is well documented that high concentrations of red blood cells inhibit growth of cells from amniotic fluid by restricting colony size. This product will not prevent maternal contamination from occurring because it has no effect on white blood cells.



Applications & Benefits
Selectively lyses unwanted red blood cells from blood-stained AF and BM samples
Non - toxic so remaining cells can be cultured
Erythrocyte Lysis Solution is a simple pretreatment of heavily blood-stained amniotic fluid samples which quickly removes heavy red blood cell (erythrocyte) contamination. Unlike may similar products used in molecular biology that lysis red blood cells, this product is extremely gentle in its action and is specially suited for cell culture work. It is well documented that high concentrations of red blood cells inhibit growth of cells from amniotic fluid by restricting colony size. This product will not prevent maternal contamination from occurring because it has no effect on white blood cells.


Ordering Information
Erythrocyte Lysis Solution - Vial of saturated liquidGGS-JL009347,00 €

Technical Specification

Procedure

see ordering details
1.To make the working solution aseptically dissolve the supplied stock solution in sterile water up to 1 litre. Mix well before use.
2.NOTE: Erythrocyte Lysis Solution is a supersaturated solution and there may be solid particles visible in the concentrated stock solution. These will
3.Centrifuge 10ml of amniotic fluid for 10 minutes at 900-1000rpm.
4.Remove supernatant aseptically and resuspend in 2 to 3ml of
5.Erythrocyte Lysis Solution and mix gently.
6.Centrifuge at 1500 rpm for 10 minutes.
7.Remove supernatant and wash in 3 mls of culture media to remove any residual Erythrocyte Lysis Solution.
8.Centrifuge again then resuspend in your medium of choice and set up in culture
Applications & Benefits
Ordering Information
Technical Specifications




Alternative content

Get Adobe Flash player



Downloads
PDF-FileProCell_Brochure.pdf
Brochure
0.49 MB
PDF-FileProcell A4 leaflet.pdf
Flyer
1.88 MB
PDF-FileErethrocyte_Lys_Sol_...
MSDS
0.18 MB


Most Wanted
Cytoclear Cytoplasm RemoverCytoclear Cytoplasm Remover
G-BandingG-Banding
FISH FFPEFISH FFPE
Anticlotting ReagentAnticlotting Reagent
CytoBrite DuoCytoBrite Duo
CytoBrite Slide OvenCytoBrite Slide Oven
Optimal Hypotonic SolutionOptimal Hypotonic Solution
Buffered Hypotonic SolutionBuffered Hypotonic Solution
Pre-Hypotonic SolutionPre-Hypotonic Solution
Erythrocyte Lysis SolutionErythrocyte Lysis Solution
Imprint | Contactform