Isoelectric Focusing


Isoelectric Focusing (IEF) is an ingenious process for simultaneous concentration and separation of proteins. IEF employs a pH gradient formed by small amphoteric molecules (ampholytes) to resolve proteins according to their different pI (isoelectric point) values. IEF is an end-point method; when the electrophoresis run is completed proteins will appear as separate sharp zones, in the order of their isoelectric point.

SERVALYTTM Carrier Ampholytes

SERVALYTTM Carrier Ampholytes are low molecular weight molecules of zwitterionic character. They are a mixture of synthetically derived species of average molecular weight distribution of 400 to 1000 Da and comprise a multitude of varying pI values. In agarose and polyacrylamide gels containing ampholytes, a linear pH gradient is generated when an electric field is applied. The ampholyte molecules carry a net charge and thus migrate in the electric field between the electrodes until they reach the position of their corresponding pI, when they stop moving and form small plateaus (stationary stacks).



Benefits
High resolution due to multimeric composition
Fast staining and destaining times
Clear background associated with very low unspecific binding of dyes and stains
High solubility in trichloroacetic acid (fast removal of ampholytes during fixation)
Virtually no interaction with metal ions


Ordering Information
pH range 2-4 42902.01 10 ml please contact us
pH range 2-4 42902.02 25 ml please contact us
pH range 2-9 Seed Mix 42935.01 10 mlplease contact us
pH range 2-9 Seed Mix 42935.02 25 ml please contact us
pH range 2-9 Seed Mix 42935.03100 ml please contact us
pH range 2-11 42900.01 10 ml please contact us
pH range 2-11 42900.02 25 ml please contact us
pH range 3-4 42922.01 10 ml please contact us
pH range 3-4 42922.02 25 ml please contact us
pH range 3-5 42903.01 10 ml please contact us
pH range 3-5 42903.02 25 ml please contact us
pH range 3-6 42944.01 10 ml please contact us
pH range 3-6 42944.01 25 ml please contact us
pH range 3-7 42945.01 10 ml please contact us
pH range 3-7 42945.01 25 ml please contact us
pH range 3-10 42940.01 10 ml please contact us
pH range 3-10 42940.02 25 ml please contact us
pH range 3-10 Iso-Dalt * 42951.01 10 ml please contact us
pH range 3-10 Iso-Dalt * 42951.01 25 ml please contact us
pH range 4-5 42923.01 10 ml please contact us
pH range 4-5 42923.02 25 ml please contact us
pH range 4-6 42904.01 10 ml please contact us
pH range 4-6 42904.02 25 ml please contact us
pH range 4-7 42948.01 10 ml please contact us
pH range 4-7 42948.02 25 ml please contact us
pH range 4-9 T ** 42910.01 10 mlplease contact us
pH range 4-9 T ** 42910.03 100 ml please contact us
pH range 4-9 T ** 42910.02 25 ml please contact us
pH range 5-6 42924.01 10 ml please contact us
pH range 5-6 42924.02 25 ml please contact us
pH range 5-7 42905.01 10 ml please contact us
pH range 5-7 42905.02 25 ml please contact us
pH range 5-7 PGM 42936.01 10 ml please contact us
pH range 5-7 PGM 42936.02 25 ml please contact us
pH range 5-8 42949.01 10 ml please contact us
pH range 5-8 42949.02 25 ml please contact us
pH range 5-9 42950.01 10 ml please contact us
pH range 5-9 42950.02 25 ml please contact us
pH range 6-7 42925.01 10 ml please contact us
pH range 6-7 42925.02 25 ml please contact us
pH range 6-8 42906.01 10 ml please contact us
pH range 6-8 42906.02 25 ml please contact us
pH range 6-9 42913.01 10 ml please contact us
pH range 6-9 42913.02 25 ml please contact us
pH range 7-9 42907.01 10 ml please contact us
pH range 7-9 42907.02 25 ml please contact us
pH range 9-11 42909.01 10 ml please contact us
pH range 9-11 42909.02 25 ml please contact us
Benefits
Ordering Information


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