The TV100YK and TV400YK-2DIEF-SYS Units: Dedicated 2-D Electrophoresis Systems


Scie-Plas now provide the first of a series of innovative solutions for scientists who prefer the latest advances in IPG (immobilised pH gradient) strip technology to more traditional capillary gels as the first step of 2-D electrophoresis. Our recently improved flatbed isoelectric focusing system (IEF-SYS) allows 1st dimension 2-D electrophoresis to be performed with multiple IPG strips within the same tank, while 2nd dimension SDS-PAGE is carried out in our existing TV100 and 400 Mini- and Maxi-gel formats (TV100YK or TV400YK).

Scie-Plas offer the complete 2-D electrophoresis system with everything you need for running reproducible 2-D gels.

IEF-SYS FOR THE 1ST DIMENSION


A recently redesigned IEF-SYS unit where a maximum of 30 SERVA IPG strips, each 3mm wide and up to 24cm in length, may be run simultaneously on a 27 x 27cm ceramic cooling plate.

FOR THE 2ND DIMENSION

Either the TV100YK twin-plate 10 x 10cm (W x H) mini-gel unit Or the TV400YK twin-plate 20.5 x 20cm (W x H) maxi-gel unit

EV232 High-Voltage 3kV 8

The Consort EV232 8 - 3000V, 150mA, 150W

FL300 Chiller Unit

Maintains cooling plate temperature during IEF

2-D ELECTROPHORESIS: OUTLINE PROTOCOL

1. Incubate the IPG strips overnight in a rehydration tray with buffer (e.g. 8M Urea, 1% CHAPS, 13mM DTT and 0.5% SERVALYT? 3-10, corresponding to the pH gradient of the IPG strip), overlaid with silicone oil, containing the protein of interest.

2. Connect the ceramic cooling plate of the IEF-SYS to the external chiller preset at 20°C, before placing the glass electrode frame directly on the cooling plate. Apply a thin layer of silicone oil over the glass plate before laying out the IPG strips at least 3mm apart.

3. After covering the anode and cathode ends of the IPG strips with electrode wicks saturated with deionised water, clip each electrode into its respective position within the glass electrode frame, before overlaying the glass electrode weight.

4. Cover the IPG strips with silicone oil to protect them from heat desiccation during IEF.

5. After IEF incubate the IPG strips for 10 minutes in Equilibration Buffer 1 (50mM Tris-HCl, pH 8.8, 6M Urea, 30% glycerol, 2% SDS, 0.01% bromophenol blue and 1% (w/v) DTT) by placing the rehydration tray on to an agitating platform. Repeat this for a further 10 minutes with Equilibration Buffer 2 (50mM Tris-HCl, pH 8.8, 6M Urea, 30% glycerol, 2% SDS, 0.01% bromophenol blue and 5% (w/v) iodoacetamide).

6. Dip each strip briefly in 1 x Laemmli Buffer before aligning it by its 1mm thick edge along the top of a pre-made 10% acrylamide gel.

7. Having covered each strip with 0.5% agarose solution, perform second-dimension SDS-PAGE .



Benefits
Glass electrode frame sits flush with the ceramic cooling plate for optimal cooling efficiency
Positive and negative electrodes made from clear acrylic clip neatly within a glass electrode frame that allows the voltage gradient to be finetuned along the entire length of each IPG strip
Glass electrode support slab ensures that the electrodes remain in complete contact with the positive and negative ends of each IPG strip during IEF


Ordering Information

Complete System

TV100YK + IEF-SYS + EV232 + external chiller unitTV100YK-2D-IEF-SYS5.891,00 €

Component Parts

Twin-plate mini-gel unit with PAGE GRM and cooled gel tank, lid, 2 x (10 x 10cm; W x H) plain glass plates, 2 x (10 x 10cm) notched glass plates, 1 x dummy plate, 4 x 1mm spacers, 4 x 1.5mm spacers, 2 x spacer aligners, 2 x 1mm thick 1-D combs, 2 x 1.5mm thick 1-D combs, casting base, 2 x silicone seals, 1 x dummy plate, 2 x quick-fit tubes, 2 x 4mm power cables and 2 x 4/2mm power output adaptorsTV100YK797,00 €Add to your cart
Flat-bed isoelectric focusing unit includes running tank, lid, 1 x ceramic cooling plate, 1 x glass plate, electrode frame, 1 x anode electrode, 1 x cathode electrode, 1 x glass electrode weight, 2 x quick-fit tubes and 2 x 2mm power cablesIEF-SYS1.506,00 €
Consort EV232 3000V, 150mA, 150W Power SupplyEV2321.230,00 €Add to your cart
Recirculating ChillerFL300please contact us

Complete System

TV400YK + IEF-SYS + EV232 + external chiller unitTV400YK-2D-IEF-SYS6.452,00 €Add to your cart

Component Parts

Twin-plate maxi-gel unit with PAGE GRM and cooled gel tank, lid, 2 x (20.5 x 20cm; W x H) plain glass plates, 2 x (20.5 x 20cm) notched glass plates, 1 x dummy plate, 4 x 1mm spacers, 4 x 1.5mm spacers, 2 x spacer aligners, 2 x 1mm thick 1-D combs, 2 x 1.5mm thick 1-D combs, casting base, 2 x silicone seals, 1 x dummy plate, 2 x quick-fit tubes, 2 x 4mm power cables and 2 x 4/2mm power output adaptorsTV400YK1.227,00 €
Flat-bed isoelectric focusing unit includes running tank, lid, 1 x ceramic cooling plate, 1 x glass plate, electrode frame, 1 x anode electrode, 1 x cathode electrode, 1 x glass electrode weight, 2 x quick-fit tubes and 2 x 2mm power cablesIEF-SYS1.506,00 €Add to your cart
Consort EV232 3000V, 150mA, 150W Power SupplyEV2321.230,00 €
Recirculating ChillerFL300please contact us
Benefits
Ordering Information


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