TV200Y Standard Twin-Plate Wide Format Mini-Gel Electrophoresis Unit


The TV200Y twin-plate 20.5 x 10cm (W x H) wide format mini-gel units allows more samples to be screened on the same gel, eliminating the problems associated with comparing samples from 2 separate mini-gels. This unit includes, as standard., a precision engineered casting base, which acts in conjunction with our recently modified TV200-GRM to provide simple, leak-free casting.

SDS-PAGE METHOD

SDS-PAGE allows proteins to separate on the basis of their molecular weight (MW) and is dependent on the action of SDS - a detergent that equalises the charge to mass ratio of each protein by coating it with a uniform negative charge - and the sieving action of the polyacrylamide gel matrix. This technique is particularly useful for determining the MW of individual protein subunits and as the first step of western blotting to examine the post-translational modification of individual proteins. Scie-Plas recommends using high quality chemicals in the application of this technique and their part numbers are shown.

1. Clean glass plates with distilled water and methanol.

2. Tape 3 sides of each gel cassette to form a leak-free seal and insert into the GRM (see diagram
below for tape-cast gels).

3. Prepare and pour a 10% resolving gel as follows for two 10 x 10cm:

Resolving gelPartNo.
7.5ml 40% (37.5:1) acrylamide(10681.01)
11.25ml 1M Tris-HCl, pH 8.5(39794.01)
0.15ml 20% (w/v) SDS(20767.03)
0.1ml 10% (w/v) APS(13375.05)
0.02ml TEMED(35925.01)
11.0ml distilled water

4. Overlay distilled water on top of the gel, allowing the gel to set for 30 minutes.

5. Remove the water and prepare and pour a 4.5% stacking gel as follows:


Stacking gelPart No.
1.15ml 40% (37.5:1) acrylamide(10681.01)
1.25ml 1M Tris-HCl, pH 6 (39794.01; to pH 6 with HCl)
0.05ml 20% (w/v) SDS (20767.03)
0.03ml 10% APS(13375.05)
0.01ml TEMED(35925.01)
7.5ml distilled water-

6. Insert the comb and allow the gel to set.

7. Mix the protein samples with Tris-Glycine/SDS sample buffer (2x) buffer (Tris/HCl pH 6, 126mM, glycerol 20%, SDS 4%, bromophenol blue 0.02%; 42527.01) and denature for 2 minutes at 98°C and then centrifuge for 2 minutes at 14000rpm.

8. Fill the inner buffer chamber and tank with Tris-Glycine/SDS electrophoresis buffer (10x)
(0.25M Tris, 1.92M Glycine and 1% SDS; 42529.01).

9. Load the protein samples into the wells according to the well volumes in aqueous solution.

10. Run the gel according to the operating guidelines in Technical Information.

11. After electrophoresis, turn off the 8 and remove the GRM from the tank after
emptying it of buffer.

12. Remove the plates from the GRM and gently prise them open with a spacer at a corner but
NOT at the notched ears - the gel is now ready for the next application e.g. Western Transfer,
staining etc.



Benefits
Twin-plate 20.5 x 10cm (W x H) wide format mini-gel unit designed specifically for Page techniques requiring self-cast gels
Wide format 20.5cm gel width effectively allows 2 mini-gels to be run within the same gel
Casting base included as standard
Glass plate stops within the newly modified GRM - lock the glass plates firmly into position onto soft silicone gaskets in the casting base to ensure leak-free gelcasting
Additional gel casting with the optional TV400-MC2 and 2 gel multicasters
Float glass plates 4mm thick - guarantee uniform gel thickness and even sample migration, and with ground edges to inhibit sample leakage during casting
Twin screws on each gel-clamp - act in conjunction with a counterbalanced, dovetailed silicone gasket to disperse pressure evenly along the height and breadth of the gel as the screws are tightened
Colour coded combs and spacers available in 0.75, 1, 1.5 and 2mm thicknesses


Ordering Information
Twin-plate wide format mini-gel unit with GRM and gel tank, lid, 2 x (20.5 x 10cm; W x H) plain glass plates, 4 x 1mm spacers, 2 x spacer aligners, 2 x (20.5 x 10cm) notched glass plates, 1 x dummy plate, 2 x 1mm thick 24-sample combs, casting base and 2 x silicone sealsTV200Y1.595,00 €

Technical Specification

TV200Y

see ordering details
Unit Dimensions (WxDxH)30x15x14cm
Inner Tank Dimensions (WxDxH)27x11x11.5cm
Plate Dimensions (WxHxT)20.5x10x0.4cm
Standard Spacer Dimensions (WxHxT)2x20x0.1cm
Active Gel Dimensions (WxH)16.5x8.5cm
Maximum Sample Capacity2 x 48
Recommended Buffer Volume
Inner Buffer Chamber300ml
Gel Tank2800ml
Recommended Running Conditions for Denaturing/Native PAGE Gel
Voltage100 - 150V
(10- 15V/cm)
Current10 - 15mA
Time1.5 - 2h
Snap-lock Connectors for Cooling Coil
Inner Diametern/a
Outer Diametern/a
Quick-fit Tubing
Inner Diametern/a
Outer Diametern/a
Casting Base Silicone Seal Dimensions (WxLxH) 1.7x22.5x0.8cm
Power Output Connectors (diameter)Shrouded, 4mm
Recommended Power SuppliesConsort EV243


Combs
Order-NrThickness(mm)Sample ThroughputTooth Widt (mm)Tooth Height (mm)Max. Spacing (mm)Sample Volume
in a 5mm
Deep Weel (µl)
PriceAdd
TV400-C0.35-240,35244,75528please contact us
TV400-C0.35-36MC0,35362,5524please contact us
TV400-C0.75-18MC0,7518620390please contact us
TV400-C0.75-240,75244,7520271please contact us
TV400-C0.75-36MC0,75362,520237please contact us
TV400-C0.75-480,75482,3520135please contact us
TV400-C1-18MC1186203120please contact us
TV400-C1-241244,7520295please contact us
TV400-C1-36MC1362,520250please contact us
TV400-C1-481482,3520147please contact us
TV400-C1.5-18MC1,5186203180please contact us
TV400-C1.5-241,5244,75202142please contact us
TV400-C1.5-36MC1,5362,520275please contact us
TV400-C1.5-481,5482,3520170please contact us
ORDERNR-MC = Multichannel pipette compatible
Benefits
Ordering Information
Technical Specifications
Combs


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