Electrophoresis Reagents and Buffers


AGAROSE GEL REAGENTS AND BUFFERS

This range of products has been continuously expanded and offers a variety of reagents for electrophoresis, including solid gel media, gel solutions, precast gels, ready-to-use buffers and solutions, as well as stains, dyes and markers.

Agarose is a highly purified, naturally-occurring polysaccharide. The preparation of agarose gels involves simply heating the powdered agarose in buffer to dissolve it. It will then gel upon cooling. Like acrylamide the pore size of an agarose gel is inversely dependent on the agarose concentration. The pores in agarose gels are generally much larger than those in acrylamide gels, making them suitable for the separation of much larger nucleic acid fragments. There are many types of agarose available. The best choice for routine DNA electrophoresis is Agarose for DNA Electrophoresis (Catalogue Number 11404). This offers good gel strength and low impurities that might interfere with subsequent procedures.

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